Detection and molecular characteristics of methicillin resistant staphylococcus aureus MRSA in some meat

Document Type : Original Article

Author

قسم البكتيريا والفطريات والمناعة كلية الطب البيطري جامعة المنوفية

Abstract

Food poisoning is one of the most important problems that can affect large part of population. Staphylococcus aureus (S. aureus) is one of the main causes of bacterial food poisoning. A hundred random samples of burger, chicken nuggets, luncheon and kofta (25 of each) obtained from retail outlets were examined bacteriologically for the occurrence of methicillin resistance Staphylococcus aureus (MRSA). A total of 25 S.aureus isolates was recovered from - burger 9/25 (36%), chicken nuggets 7/25 (28%), luncheon 6/25(24 %) and kofta 3/25 (12%) Application of antibiotic sensitivity test on isolated S.aureus cleared that the highest resistance was exhibited against penicillin (96%) followed by flucloxacillin (92%), cefotaxime (68%), erythromycin (60%), clindamycin (61.54%), linezolid (36%) and vancomycin (28%) , using Polymerase chain reaction (PCR) on S.aureus to detect their virulence and resistance genes Hemolysin virulence gene (hlg) which was detected in (50%) of the examined isolates, while beta- lactam resistance gene (blaZ) and met hicillin resistance gene (mec A) were found in (50%) and (100%) of tested S.aureus, respectively.
KEYWORDS: S.aureus, Burger, meat products, Resistant genes.

Keywords

Menoufia Veterinary Medical Journal
Volume 1, Issue 1 - Serial Number 1
المجلد الاول العدد الاول يونيو 2025
August 2025
Pages 1-12

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