Lactating Cattle Subclinical Mastitis: Comparative Efficacy of Different Diagnostic Tests and Associated Risk Factors

Document Type : Original Article

Authors

1 infectious diseases menofia veternairy medicen

2 food hygiene, animal health research institute,ARC, Egypt

3 Professor of Infectious Diseases, Head of Medicine and Infectious Diseases Department. Faculty of Vet Medicine, EL Sadat University

4 Department of Pathology, Faculty of Veterinary Medicine, Menoufia University

5 Professor of Infectious Diseases and Vice Dean For Student Affairs. Faculty of Vet Medicine, Menoufia University

Abstract

Subclinical mastitis (SCM) is a prevalent infection of the udder that causes significant economic losses in the dairy sector on a global scale. The primary objective was to assess and contrast the clinical efficacy of three screening tests that are currently available for identifying SCM. A total of 400 foremilk samples were obtained from 100 Lactating cattle in Menoufia governorate between May 2022 and April 2023. The diagnosis of mastitis was conducted by utilizing bacterial cultures of foremilk samples as a reference, which involved isolating the causal pathogens. For mastitis diagnosis in the field, the California Mastitis Test (CMT) was used. The BacSomatic® method was used to estimate the somatic cell count (SCC). Additionally, the pH of the milk was determined on-site using a pH meter. The performance of the tests was assessed by determining the test sensitivity, specificity, and accuracy at the appropriate cut-off point for each test. The BacSomatic® test demonstrated exceptional performance in identifying mastitis, with a sensitivity of 98.3%, a specificity of 96.8%, and an accuracy of 97.7% at a cut-off point of >200,000 cells/mL. When comparing several tests, it is found that CMT is the second most effective test when using an ideal cut-off point for a score that is not negative. This test has a sensitivity of 84.7%, a specificity of 85.3%, and an accuracy of 85%. The pH meter demonstrated satisfactory test performance, with a sensitivity of 80.9%, a specificity of 81.5%, and an accuracy of 81.3%.

Keywords

Menoufia Veterinary Medical Journal
Volume 1, Issue 1 - Serial Number 1
المجلد الاول العدد الاول يونيو 2025
August 2025
Pages 1-15

Files

Share

How to cite

Statistics