Document Type : Original Article
Authors
1
Department of Milk Hygiene and Control, Faculty of Veterinary Medicine, Menoufia University, Egypt
2
Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Menoufia University
3
Asst. Lecturer , of milk Hygiene and Control Department Faculty of Veterinary Medicine shebien Elkome– EL Monofia University
Abstract
Finding the prevalence and antimicrobial sensitivity of Escherichia Coli in uncooked milk and some dairy products (soft cheese, Karish cheese) was the goal of the current study. Eosine methylene blue agar (EMB) was used to test 85 samples of uncooked milk and dairy products for the presence of Escherichia Coli. Following biochemical confirmation of suspected Escherichia Coli isolates, a subset of Escherichia Coli strains were identified serologically. Additionally, strains that were identified by serology underwent PCR after being tested for antimicrobial sensitivity. In the samples that were analyzed, the incidence of Escherichia Coli was 14.3%, 12%, and 32%, respectively. O26:H11, O44:H18, O55: H7, O159, O91:H21, O111:H2, O117:H4 and O128:H2 were the serologically identified organismic strains of Escherichia Coli that were recovered from milk samples. High resistance to Penicillin (87.5%), Solphamethoxzol (68.8%), Nalidixic acid (62.5%), and Ampicillin and Tetracycline (50%) is revealed by the antimicrobial susceptibility results. On the other hand, the highest susceptibility was to Ampicillin (31.3%), Tetracycline (37.5%), Ciprofloxacin (56.3%), and Gentamicin (75%). In order to prevent harmful and toxic Escherichia Coli, effective hygiene measures are necessary.
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